Financed by
Ana Martínez del Val
Laboratory of Cardiovascular Proteomics, Centro Nacional de INvestigaciones Cardiovasculares (CNIC), CIBER de Enfermedatdes Cardiovasculares (CIBERCV), Madrid, Spain
Proteomics strategies to study post-translational regulatory mechanisms in cell biology and disease
Cells respond to environmental cues, such as external signal or stresses, by dynamically remodeling their proteome. A great part of the proteome-level regulation within a cell happens post-translationally. Post-translational regulation can involve chemical modifications in the protein, but also subcellular relocation, secretion or targeted degradation. Mass spectrometry-based proteomics offers an exceptional platform to explore from a systems-level perspective all these dynamic mechanisms. In this talk, I will present how using MS-based proteomics we can study cell response and adaptation to stress from a post-translational perspective, either by studying the spatio-temporal dynamics within the cells or their modification status.
Selected references
1. P. Bortel, et al. Systematic Optimization of Automated Phosphopeptide Enrichment for High-Sensitivity Phosphoproteomics. Molecular & Cellular Proteomics 23 (2024)
2. U. H. Guzman, et al. Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition. Nat Biotechnol 42, 1855–1866 (2024)
3. A. Martínez-Val, et al. Hybrid-DIA: intelligent data acquisition integrates targeted and discovery proteomics to analyze phospho-signaling in single spheroids. Nat Commun 14, 3599 (2023).
This seminar is supported by the B2S Federation
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